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Alexander
de Luna Fors Department of Systems Biology 200 Longwood Ave, Alpert Bldg Tel. +1 (617) 432-6393 Fax +1 (617) 495-2196 adeluna [at] hms.harvard.edu |
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Home Background
Past
and Current Research Publications Awards Research
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talks Teaching
experience
Updated: June 2007 |
BACKGROUND I grew
up in I am
a Postdoctoral Fellow in Roy Kishony’s lab at the Department of Systems Biology in
Harvard Medical School (previously at the Bauer Center for Genomics Research in
Harvard University). Here, I am enjoying the most working closely with
and learning from my physicists collaborators. PAST AND
CURRENT RESEARCH My
doctoral training was strongly focused in biochemistry and yeast genetics. We
defined a role for gene redundancy in the pathways for glutamate
biosynthesis, describing for the first time the functional relevance of a
gene duplication event at the transcriptional and enzymatic level in yeast. I
later conducted a short postdoc at IFC, where I
collaborated in the study of interactions between transcription factors in
amino acid catabolism and biosynthesis genes using DNA microarrays.
During this time, I was also involved in undergrad teaching and Theses
mentoring. I
currently use a combination of theoretical and experimental approaches to
study genetic interactions—interactions which illustrate how mutations affect
the phenotypic consequences of each other. We first used a flux balance model
to simulate the genetic interaction network of yeast and extended the concept
of genetic interaction from the gene-gene level to the system level. More
recently, we have developed an automated experimental tool based on
fluorescence measurements to achieve high-throughput, yet very accurate,
quantification of phenotypes in yeast. Using this powerful phenotyping methodology, we plan to study further aspects
of the structural and functional design of genetic networks. PUBLICATIONS From my work in ·
D Segrè, A DeLuna, GM Church, R Kishony. (2005)
Modular epistasis in yeast metabolism. Nature Genetics
37(1):77-83. [link] From my work in · A
DeLuna, H Quezada, A Gomez-Puyou, A Gonzalez. (2005) Asparaginyl
Deamidation in Two Glutamate Dehydrogenase
Isoenzymes from Saccharomyces
cerevisiae. Biochemical and
Biophysical Research Communications 328(4):1083-1090. [link] ·
A DeLuna, A Avendaño, L Riego, A González. (2001)
NADP-glutamate dehydrogenase isoenzymes of Saccharomyces cerevisiae.
Purification, enzyme kinetics and physiological roles. Journal of Biological Chemistry 276(47):43775–43783. [link] ·
C Ishida, C Aranda, L Valenzuela, L Riego, A DeLuna,
S Zonszein, F Recillas-Targa, A González. (2005) The UGA3-GLT1 intergenic
region of Saccharomyces cerevisiae:
A model to study the combinatorial action of Gln3p, Gcn4p and chromatin
organization on the transcriptional activity of a bidirectional promoter. Molecular
Microbiology 59(6):1790-1806. ·
A Avendaño, L Riego, A
DeLuna, C Aranda, M Vázquez-Acevedo, F Recillas-Targa, L Valenzuela, ·
C
Aranda, M Colon, C Ishida, L Riego, A DeLuna, L
Valenzuela, J Herrera, A Gonzalez. (2006) Gcn5p contributes to the bidirectional character of the UGA3-GLT1 yeast promoter. Biochemical and Biophysical Research Communications
348(3):989-996. ·
CA
Guerrero, C Aranda, A DeLuna, P Filetici, L Riego, VH Anaya, González.
(2005) Salt-dependent expression of ammonium assimilation genes in the
halotolerant yeast Debaryomyces hansenii. Current Genetics 47(3):163-171. · L Alba-Lois, C Segal, B Rodarte, V Valdés-López, A DeLuna, R
Cárdenas. (2004) NADP-glutamate dehydrogenase activity is
increased under hyperosmotic conditions in the halotolerant yeast Debaryomyces hansenii. Current Microbiology 48 (1):68-72. ·
· L Riego, A Avendaño, A DeLuna, E Rodríguez, A
González. (2002) GDH1 expression is regulated by GLN3,
GCN4 and HAP4 under respiratory growth. Biochemical and
Biophysical Research Communications 293(1):79–85. · A Avendaño, A DeLuna, L Valenzuela, H Olivera,
A González. (1997) GDH3 encodes a
glutamate dehydrogenase isozyme, a previously
unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae. Journal
of Bacteriology 197(17):5594–5597. AWARDS · Pew
Postdoctoral Fellow. Pew Latin American Fellows Program in the Biomedical
Sciences ( · Member
of the Sistema Nacional de Investigadores, Nivel I ( RESEARCH
VISITS ABROAD · MRC
Centre for Protein Engineering. INVITED
TALKS ·
9th Annual Plant Sciences Symposium. Epistasis: Defining Phenotypes and Evolutionary Trajectories. Iowa State University. Ames, Iowa. June 1,
2007 ·
XXIX Congreso Nacional de Genética Humana. Asociación
Mexicana de Genética Humana. · Instituto de Fisiología Celular, Universidad Nacional Autónoma de México.
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Départment
Structure et Dynamique des Génomes, Institut Pasteur. ·
USDA-ARS
Center for Agricultural Bioinformatics, Cornell University. Ithaca, NY, USA. Nov 12, 2003 · Centro de Investigación
en Biotecnología, Universidad Autónoma del Estado de Morelos. ORGANIZATION
OF SCIENTIFIC MEETINGS ·
Evolutonary Genomics in Modern Integrative Biology (School and Symposium). Pátzcuaro, Mexico. 8 - 14 Oct
2006 ·
Bioinformatics, Genomics and Proteomics (School and
Symposium). Puerto Vallarta,
Mexico. 14 - 16 Mar 2004 · Evolutionary and Comparative Genomics (School and
Symposium). Puerto Vallarta,
Mexico, 29 Oct - 4 Nov
2002 TEACHING
EXPERIENCE ·
Molecular Biology of the Cell I and II
(Biochemistry and Metabolism). Facultad de Ciencias, UNAM (undergraduate). June 2001 – September
2003. · Senior Thesis Mentorting (undergraduate). Victor
Hugo Anaya Muñoz. Biología.
Facultad de Ciencias,
UNAM. September 2002. · Master’s Thesis Mentorting. Victor Hugo Anaya Muñoz. Graduate Program in Experimental Biology. Facultad de Ciencias, UNAM. May
2005.
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